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6Multiphoton-targeted photochemistry was used to selectively inactivate the expression of genes in vertebrate cells. A membrane permeable DNA-associating vital dye, ethidium bromide monoacetate was used to photosensitize chromosomes in dividing cells. A 100-ps infrared laser beam operating at 1.06 microns was focused onto a selected region of a mitotic chromosome corresponding to the sites of the nucleolar genes. Individual cells followed through mitosis demonstrated a reduction in the number of …Read more
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University of California, Santa BarbaraDepartment of Philosophy, University of California, Santa BarbaraGraduate student
Santa Barbara, California, United States of America
Areas of Interest
Metaphilosophy |
Philosophy of Language |