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    Marsilio Ficino’s fame as a translator, not least due to his contributions to theology and the development of hermeticism, has already been established by Frances Yates and debated by Wouter Hanegraaff. For each of his translations of Greek texts, Ficino wrote a preface to guide and to manipulate the reader. This paper presents an analysis of the auctoritas in the paratext of the Corpus Hermeticum, analyzing it as a rhetorical device used by Ficino to express his ideas, particularly the role of …Read more
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    In situ characterization of CD4 + T cell behavior in mucosal and systemic lymphoid tissues during the induction of oral priming and tolerance
    with B. H. Zinselmeyer, J. Dempster, A. M. Gurney, D. Wokosin, M. Miller, H. Ho, O. R. Millington, K. M. Smith, C. M. Rush, M. Cahalan, J. M. Brewer, and P. Garside
    The behavior of antigen-specific CD4+ T lymphocytes during initial exposure to antigen probably influences their decision to become primed or tolerized, but this has not been examined directly in vivo. We have therefore tracked such cells in real time, in situ during the induction of oral priming versus oral tolerance. There were marked contrasts with respect to rate and type of movement and clustering between naive T cells and those exposed to antigen in immunogenic or tolerogenic forms. Howeve…Read more
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    Oligomeric forms of Aβ peptides are implicated in Alzheimer's disease and disrupt membrane integrity, leading to cytosolic calcium elevation. Proposed mechanisms by which Aβ mediates its effects include lipid destabilization, activation of native membrane channels, and aggregation of Aβ into Ca2+-permeable pores. We distinguished between these using total internal reflection fluorescence microscopy to image Ca2+ influx in Xenopus laevis oocytes. Aβ1-42 oligomers evoked single-channel Ca2+ fluore…Read more
  • Two-photon imaging has revealed an elegant choreography of motility and cellular interactions within the lymph node under basal conditions and at the initiation of an immune response 1. Here, we present methods for adoptive transfer of labeled T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph node as first described in 2002 2. Two-photon imaging of immune cells requires that the cells are fluorescently labeled, either by staining with a cell tracker d…Read more