Trevor Anderson

This article examines the role that humbling plays in Socratic practice. Specifically, we consider how Socrates humbles his interlocutors in order to turn them towards the pursuit of philosophical friendship. We argue against a standard interpretation of humbling in the Lysis, which holds that Socrates humbles Lysis by exposing his own ignorance to him at 210d. Instead, we argue that the humbling occurs not when Lysis is (allegedly) made aware of his own ignorance, but at 222d near the end of the dialogue, when Lysis is made to think that he is not as good a friend as he thought he was. On this reading, Socrates humbles Lysis not by exposing to him his ignorance about theoretical matters but by suggesting to him that he may be not the sort of person he thought he was.

Improved tools for providing specific intraoperative diagnoses could improve patient care. In neurosurgery, intraoperatively differentiating non-operative lesions such as CNS B-cell lymphoma from operative lesions can be challenging, often necessitating immunohistochemical procedures which require up to 24-48 hours. Here, we evaluate the feasibility of generating rapid ex vivo specific labeling using a novel lymphoma-specific fluorescent switchable aptamer. Our B-cell lymphoma-specific switchable aptamer produced only low-level fluorescence in its unbound conformation and generated an 8-fold increase in fluorescence once bound to its target on CD20-positive lymphoma cells. The aptamer demonstrated strong binding to B-cell lymphoma cells within 15 minutes of incubation as observed by flow cytometry. We applied the switchable aptamer to ex vivo xenograft tissue harboring B-cell lymphoma and astrocytoma, and within one hour specific visual identification of lymphoma was routinely possible. In this proof-of-concept study in human cell culture and orthotopic xenografts, we conclude that a fluorescent switchable aptamer can provide rapid and specific labeling of B-cell lymphoma, and that developing aptamer-based labeling approaches could simplify tissue staining and drastically reduce time to histopathological diagnoses compared with IHC-based methods. We propose that switchable aptamers could enhance expeditious, accurate intraoperative decision-making.