Wu Hsiang-Yun

Clinical computed tomography provides a single mineral density value for heterogeneous calcified tissues containing early and late stage pathologic formations. The novel aspect of this study is that, it extends current quantitative methods of mapping mineral density gradients to three dimensions, discretizes early and late mineralized stages, identifies elemental distribution in discretized volumes, and correlates measured MD with respective calcium to phosphorus and Ca to zinc elemental ratios. To accomplish this, MD variations identified using polychromatic radiation from a high resolution micro-computed tomography benchtop unit were correlated with elemental mapping obtained from a microprobe X-ray fluorescence using synchrotron monochromatic radiation. Digital segmentation of tomograms from normal and diseased tissues contained significant mineral density variations. A plausible linear correlation between segmented MD volumes and elemental ratios within these volumes was established, and Ca/P ratios for dentin, hypomineralized dentin, cementum, and bone were observed. Furthermore, varying Ca/Zn ratios were distinguished in adapted compared to normal tissues, such as in bone and in cementum, highlighting Zn as an influential element in prompting observed adaptive properties. Hence, results provide insights on mineral density gradients with elemental concentrations and elemental footprints that in turn could aid in elucidating mechanistic processes for pathologic formations.