• In situ mechanotransduction via vinculin regulates stem cell differentiation
    with A. W. Holle, D. Vijayraghavan, L. G. Vincent, A. Fuhrmann, Y. S. Choi, J. C. Del Alamo, and A. J. Engler
    Human mesenchymal stem cell proliferation, migration, and differentiation have all been linked to extracellular matrix stiffness, yet the signaling pathway that are necessary for mechanotransduction remain unproven. Vinculin has been implicated as a mechanosen-sor in vitro, but here we demonstrate its ability to also regulate stem cell behavior, including hMSC differentiation. RNA interference-mediated vinculin knockdown significantly decreased stiffness-induced MyoD, a muscle transcription fact…Read more