Jose Carrillo

La ciencia moderna ha reducido el estudio de la realidad a una sola postura mostrando una tendencia reductiva de lo real solamente exclusiva al hecho contrastable de la experiencia sensible. La propuesta es mostrar una contraparte desde una perspectiva filosófica. La filosofía contemporánea, especialmente el denominado nuevo realismo ha comenzado a problematizar esta visión única de lo real. La ontología del siglo XXI permite revalorar las nociones filosóficas de ser, existencia, realidad y cuerpo. Este artículo cuestiona críticamente los resultados científicos alcanzados por una explicación de la filosofía moderna sobre el cuerpo. Una reflexión inspirada en el Cur Deus Homo de San Anselmo de Canterbury, permitirá esbozar esta alternativa crítica al entendido moderno sobre el origen de la conciencia y su corporalidad. La novedad de este pensamiento queda circunscrito al entendido ontológico medieval sobre la creatio ex nihilo, explicación que no recurre por necesidad a un reducto de lo real con sus características espacio-temporales, pues el fundamento del ser viene dado por una exterioridad de un poder creador divino, que si bien, puede no ser aceptado del todo por la filosofía y ciencia contemporánea, el nuevo realismo se ha preocupado por volver a problematizar la cuestión de Dios.

The high molecular weight glutenin subunit composition of a collection of 107 Argentinean bread wheat cultivars was analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Allelic variation at the Glu-1 loci was identified and its frequency calculated. Eleven alleles were detected, three encoded at the Glu-A1 locus, six at the Glu-B1 locus and two at the Glu-D1 locus. A low frequency of the null allele at the Glu-A1 locus and a high frequency of subunits 5+10 at the Glu-D1 locus were observed. Reversed phase-high performance liquid chromatography analysis was used to further characterise HMW-GS. Two different types of Bx subunit 8 were detected, the latter having shorter elution time. Subunit 8* was not identifiable by SDS-PAGE. According to quantification by RP-HPLC analysis, two groups of subunit 7 were observed. One group, with a relatively high proportion of subunit 7 appeared in cultivars with allele 7+8* at the Glu-B1 locus; a second group of subunit 7, was found in alleles 7+8, 7+8* and 7+9. Restriction fragment length polymorphisms analyses of HMW-GS genes were also carried out after digestion of genomic DNA with HindIII and TaqI restriction enzymes. The relationship between DNA fragment size and glutenin subunits, as estimated by electrophoretic mobility in SDS-PAGE, was also examined. The restriction enzyme TaqI demonstrated to be a useful tool to detect homozygous plants in selection programs against the Glu-A1 null allele.