Yuchen Liang

It is conventionally accepted that while Western philosophy has “being” as a central topic, Eastern thoughts focused only on “nothing”. I will challenge this perception by retrieving the original meaning of the Chinese existential word 存 cun, which can provide a hitherto neglected affective aspect of being, which in the West is also mentioned by only a handful of philosophers, including Heidegger’s famous discussion of Sorge. I will utilize Heidegger’s hermeneutical phenomenology on cun by looking into the present state of cun’s usage, comparing to its original meaning, and proposing a new way forward. Cun and its compound form cunzai is used to translate “being” in modern East Asian languages. However, recently Chinese philosophers began to question the appropriateness of using cunzai for this role. I will go back to the original meaning of cun as care with both lexicographical and orthographical evidence, comparing its antonym pairing to that of the other East Asian existential words such as 是 shi and 有 you, and trace its changes in meaning. In the last section I will propose possible changes to our dealing with our own existence when we retrieve the original affective aspect of cun, with its coherence of care and existence, through the philosophical use of cun in Mencius.

Tight control and multifactorial regulation of gene expression are important challenges in genetic engineering and are critical for the development of regulatory circuits. Meeting these challenges will facilitate transgene expression regulation and support the fine-tuning of metabolic pathways to avoid the accumulation of undesired intermediates. By employing the endoribonuclease Csy4 and its recognition sequence from Pseudomonas aeruginosa and manipulating 5'UTR of mRNA, we developed a two-component expression-repression system to tightly control synthesis of transgene products. We demonstrated that this regulatory device was functional in monocotyledonous and dicotyledonous plant species, and showed that it can be used to repress transgene expression by >400-fold and to synchronize transgene repression. In addition to tissue-specific transgene repression, this system offers stimuli-dependent expression control. Using a bioinformatics approach, we identified 54 orthologous systems from various bacteria, and then validated in planta the activity for a few of those systems, demonstrating the potential diversity of such a two-component repressor system.