Anway Mukhopadhyay

Overconsumption, which leads to considerable adverse effects on sustainability, is a significant concern in the tourism sector, especially under flat-rate pricing (e.g., buffets), where consumption is unlimited after consumers pay a fixed price. When charged with higher prices, will people consume more regardless of how much they need? Previous findings are mixed. To answer this question, by conducting three laboratory experiments and one field experiment, our research introduces an influential ideology and shows that this higher-price-driven overconsumption behavior only holds among people who believe in the Protestant Work Ethic, an influential concept used to explain the historical rise of capitalism. People who believe in the Protestant Work Ethic emphasize input-outcome correspondence and are therefore more likely to feel entitled to consume more as their expenditure increases. This belief leads them to overconsume under flat-rate pricing. In contrast, people who do not subscribe to the Protestant Work Ethic consume similar quantities regardless of their spending. Our findings extend the Protestant Work Ethic literature, revealing how this virtuous construct can lead to not-so-virtuous behavior like overconsumption. Marketers can leverage this to influence consumer overconsumption, enhancing profitability and sustainability.

Enhancing xylose utilization has been a major focus in Saccharomyces cerevisiae strain-engineering efforts. The incentive for these studies arises from the need to use all sugars in the typical carbon mixtures that comprise standard renewable plant-biomass-based carbon sources. While major advances have been made in developing utilization pathways, the efficient import of five carbon sugars into the cell remains an important bottleneck in this endeavor. Here we use an engineered S. cerevisiae BY4742 strain, containing an established heterologous xylose utilization pathway, and imposed a laboratory evolution regime with xylose as the sole carbon source. We obtained several evolved strains with improved growth phenotypes and evaluated the best candidate using genome resequencing. We observed remarkably few single nucleotide polymorphisms in the evolved strain, among which we confirmed a single amino acid change in the hexose transporter HXT7 coding sequence to be responsible for the evolved phenotype. The mutant HXT7 shows improved xylose uptake rates that allows the S. cerevisiae strain to show significant growth with xylose as the sole carbon source, as well as partial co-utilization of glucose and xylose in a mixed sugar cultivation.

Tight control and multifactorial regulation of gene expression are important challenges in genetic engineering and are critical for the development of regulatory circuits. Meeting these challenges will facilitate transgene expression regulation and support the fine-tuning of metabolic pathways to avoid the accumulation of undesired intermediates. By employing the endoribonuclease Csy4 and its recognition sequence from Pseudomonas aeruginosa and manipulating 5'UTR of mRNA, we developed a two-component expression-repression system to tightly control synthesis of transgene products. We demonstrated that this regulatory device was functional in monocotyledonous and dicotyledonous plant species, and showed that it can be used to repress transgene expression by >400-fold and to synchronize transgene repression. In addition to tissue-specific transgene repression, this system offers stimuli-dependent expression control. Using a bioinformatics approach, we identified 54 orthologous systems from various bacteria, and then validated in planta the activity for a few of those systems, demonstrating the potential diversity of such a two-component repressor system.

© 2016 The Author.Background: BAHD acyltransferases, named after the first four biochemically characterized enzymes of the group, are plant-specific enzymes that catalyze the transfer of coenzyme A-activated donors onto various acceptor molecules. They are responsible for the synthesis in plants of a myriad of secondary metabolites, some of which are beneficial for humans either as therapeutics or as specialty chemicals such as flavors and fragrances. The production of pharmaceutical, nutraceutical and commodity chemicals using engineered microbes is an alternative, green route to energy-intensive chemical syntheses that consume petroleum-based precursors. However, identification of appropriate enzymes and validation of their functional expression in heterologous hosts is a prerequisite for the design and implementation of metabolic pathways in microbes for the synthesis of such target chemicals. Results: For the synthesis of valuable metabolites in the yeast Saccharomyces cerevisiae, we selected BAHD acyltransferases based on their preferred donor and acceptor substrates. In particular, BAHDs that use hydroxycinnamoyl-CoAs and/or benzoyl-CoA as donors were targeted because a large number of molecules beneficial to humans belong to this family of hydroxycinnamate and benzoate conjugates. The selected BAHD coding sequences were synthesized and cloned individually on a vector containing the Arabidopsis gene At4CL5, which encodes a promiscuous 4-coumarate:CoA ligase active on hydroxycinnamates and benzoates. The various S. cerevisiae strains obtained for co-expression of At4CL5 with the different BAHDs effectively produced a wide array of valuable hydroxycinnamate and benzoate conjugates upon addition of adequate combinations of donors and acceptor molecules. In particular, we report here for the first time the production in yeast of rosmarinic acid and its derivatives, quinate hydroxycinnamate esters such as chlorogenic acid, and glycerol hydroxycinnamate esters. Similarly, we achieved for the first time the microbial production of polyamine hydroxycinnamate amides; monolignol, malate and fatty alcohol hydroxycinnamate esters; tropane alkaloids; and benzoate/caffeate alcohol esters. In some instances, the additional expression of Flavobacterium johnsoniae tyrosine ammonia-lyase allowed the synthesis of p-coumarate conjugates and eliminated the need to supplement the culture media with 4-hydroxycinnamate. Conclusion: We demonstrate in this study the effectiveness of expressing members of the plant BAHD acyltransferase family in yeast for the synthesis of numerous valuable hydroxycinnamate and benzoate conjugates.

This book deals with the intricate issue of approaching atheism—methodologically as well as conceptually—from the perspective of cultural pluralism. What does ‘atheism’ mean in different cultural contexts? Can this term be applied appropriately to different religious discourses which conceptualize God/gods/Goddess/goddesses (and also godlessness) in hugely divergent ways? Is my ‘God’ the same as yours? If not, then how can your atheism be the same as mine? In other words, this volume raises the question: Is it not high time that we proposed a comparative study of atheism(s) alongside that of religions, rather than believing that atheism is centered in the ‘Western’ experience? Apart from answering these questions, the book highlights the much-needed focus on the philosophical negotiations between atheism, theism and agnosticism. The fine chapters collected here present pluralist negotiations with the notion of atheism and its ethical, theological, literary and scientific corollaries.

This special issue of Sophia, titled Living without God: A Multicultural Spectrum of Atheism, deals with the intricate issue of approaching atheism—methodologically as well as conceptually—from the perspective of cultural pluralism. What does ‘atheism’ mean in different cultural contexts? Can this term be applied appropriately to different religious discourses which conceptualize God/gods/Goddess/goddesses in hugely divergent ways? Or would that rather be a sort of hegemonic homogenization of all possible modalities of living without God, as Jessica Frazier argues? Is my ‘God’ the same as yours? If not, then how can your atheism be the same as mine? In other words, this issue of Sophia raises the question: Is it not high time that we proposed a comparative study of atheism alongside that of religions, rather than believing that atheism is centered in the ‘Western’ experience? Besides, how can we explore the modalities of atheist religiosity such as we find in Buddhism and Jainism and also, arguably, in certain forms of Hinduism, as far as the Indic traditions are concerned? How might these negotiations of atheism across the multicultural spectrum interrogate our tendency to place atheism within the context of the binary opposition of science and religion? Besides, there is a need to focus on the philosophical negotiations between atheism, theism and agnosticism and the discourses that emerge from such dialogues, including that of postsecularity.

Abstract: The present paper seeks to explore the emotional structures that make human beings afraid of death in solitude, the feelings that necessitate the imagining of a peopled death, a death accompanied by fellow humans, gods, or God. In order to do this I take up the works of two great thinkers of the East and the West, and place them on a comparativist spectrum. The discussion covers many areas, including the polytheistic imaginations of ancient Greece and eighth century India, in the context of the complex interface of beauty and death in the thought of Shankaracharya, the great proponent of Advaita Vedanta in India, and Socrates, who, in the Platonic dialogues, presents a monism Shankara could have recognized well. I try to investigate the tension between decay and beauty which leads these philosophers towards a strict ascetic impulse. At the same time, I also argue that neither of them could exclude the beauty of this world altogether from their thought. Finally, I explore the ways in which we can imagine a beauty greater than death that does not let us logocentrically negate death but rather encompasses it. Rather than taking death as the closure of all possible beauty, as ultimate nothingness, can we envisage a beauty that drowns death in the sea of kalon envisioned by Diotima in the Symposium, or in the saundaryalahari, the flood of majestic beauty radiated by Shankaracharya’s Goddess? To explore these areas and to understand the interconnected issues of plurality, impermanence, beauty and truth, I draw on the twentieth century philosophers like Iris Murdoch and Sri Aurobindo as well as Socrates and Shankara. This paper finally embarks on an imaginative journey towards a peopled or crowded death, where gods or divinized humans accompany the dying person in the process of death as his/her “necessary others”(a la Adriana Cavarero). Author’s bio-note: Anway Mukhopadhyay is a doctoral research scholar at the Department of English, Banaras Hindu University, Varanasi, India. He is the author of Do You Love Me, Master?, The Place of Eros in the Master Slave Dialectic(Saarbrucken, Germany: Lambert Academic Publishing, 2010),and Bloom Again in Autumn, The Move towards an Empathetic Temporality in Yuri Medvedev’s “The Bride’s Room” (Munich: Grin Verlag, 2012) and has been published in reputed international journals in India, the USA and Australia. His areas of interest include Indian and Western philosophy, gender and literary and cultural theory. His current research centres round the polyvalent nuances of ‘planetary’ feminisms in our time. Email id: [email protected], [email protected]