Hanna Kim

© 2015 Walker et al.Background: Carbohydrate binding modules bind polysaccharides and help target glycoside hydrolases catalytic domains to their appropriate carbohydrate substrates. To better understand how CBMs can improve cellulolytic enzyme reactivity, representatives from each of the 18 families of CBM found in Ruminoclostridium thermocellum were fused to the multifunctional GH5 catalytic domain of CelE, which can hydrolyze numerous types of polysaccharides including cellulose, mannan, and xylan. Since CelE is a cellulosomal enzyme, none of these fusions to a CBM previously existed. Results: CelEcc-CBM fusions were assayed for their ability to hydrolyze cellulose, lichenan, xylan, and mannan. Several CelEcc-CBM fusions showed enhanced hydrolytic activity with different substrates relative to the fusion to CBM3a from the cellulosome scaffoldin, which has high affinity for binding to crystalline cellulose. Additional binding studies and quantitative catalysis studies using nanostructure-initiator mass spectrometry were carried out with the CBM3a, CBM6, CBM30, and CBM44 fusion enzymes. In general, and consistent with observations of others, enhanced enzyme reactivity was correlated with moderate binding affinity of the CBM. Numerical analysis of reaction time courses showed that CelEcc-CBM44, a combination of a multifunctional enzyme domain with a CBM having broad binding specificity, gave the fastest rates for hydrolysis of both the hexose and pentose fractions of ionic-liquid pretreated switchgrass. Conclusion: We have shown that fusions of different CBMs to a single multifunctional GH5 catalytic domain can increase its rate of reaction with different pure polysaccharides and with pretreated biomass. This fusion approach, incorporating domains with broad specificity for binding and catalysis, provides a new avenue to improve reactivity of simple combinations of enzymes within the complexity of plant biomass.

© 2015. American Geophysical Union. All Rights Reserved. The impacts of droughts on the Amazon ecosystem have been broadly discussed in recent years, but a comprehensive understanding of the consequences is still missing. In this study, we show evidence of a fragile hydrological equilibrium in the western Amazon. While drainage systems located near the equator and the western Amazon do not show water deficit in years with average climate conditions, this equilibrium can be broken during drought events. More importantly, we show that this effect is persistent, taking years until the normal hydrological patterns are reestablished. We show clear links between persistent changes in forest canopy structure and changes in hydrological patterns, revealing physical evidence of hydrological mechanisms that may lead to permanent changes in parts of the Amazon ecosystem. If prospects of increasing drought frequency are confirmed, a change in the current hydroecological patterns in the western Amazon could take place in less than a decade.

Shift work and overtime have been implicated as important work-related risk factors for cardiovascular disease. Many firefighters who contractually work on a 24-hr work schedule, often do overtime which can result in working multiple, consecutive 24-hr shifts. Very little research has been conducted on firefighters at work that examines the impact of performing consecutive 24-hr shifts on cardiovascular physiology. Also, there have been no standard field methods for assessing in firefighters the cardiovascular changes that result from 24-hr shifts, what we call "cardiovascular strain". The objective of this study, as the first step toward elucidating the role of very long shifts in the development of CVD in firefighters, is to develop and describe a theoretical framework for studying cardiovascular strain in firefighters on very long shifts. The developed theoretical framework was built on an extensive literature review, our recently completed studies with firefighters in Southern California, e-mail and discussions with several firefighters on their experiences of consecutive shifts, and our recently conducted feasibility study in a small group of firefighters of several ambulatory cardiovascular strain biomarkers. The theoretical framework developed in this study will facilitate future field studies on consecutive 24-hr shifts and cardiovascular health in firefighters. Also it will increase our understanding of the mechanisms by which shift work or long work hours can affect CVD, particularly through CVD biological risk factors, and thereby inform policy about sustainable work and rest schedules for firefighters.

An observation of Hume’s has received a lot of attention over the last decade and a half: Although we can standardly imagine the most implausible scenarios, we encounter resistance when imagining propositions at odds with established moral (or perhaps more generally evaluative) convictions. The literature is ripe with ‘solutions’ to this so-called ‘Puzzle of Imaginative Resistance’. Few, however, question the plausibility of the empirical assumption at the heart of the puzzle. In this paper, we explore empirically whether the difficulty we witness in imagining certain propositions is indeed due to claim type (evaluative v. non-evaluative) or whether it is much rather driven by mundane features of content. Our findings suggest that claim type plays but a marginal role, and that there might hence not be much of a ‘puzzle’ to be solved.

In the 2020 Yale Law Journal article, “Commonsense Consent,” Roseanna Sommers argues that deception is compatible with the layperson’s intuitive sense of consent. That is, unlike the canonical understanding of consent defended by legal scholars and philosophers, the notion of consent defended by the folk is not invalidated by deception. In this study, I find that while respondents do appear to attribute consent to victims of deception, they do so in a limited number of contexts – i.e., they attribute de re consent to victims of de dicto deception and attribute de dicto consent to victims of de re deception. When the two varieties of consent and deception are properly disambiguated and matched type for type however, we see the reverse phenomenon – i.e., participants refrain from attributing de dicto consent to de dicto deception and refrain from attributing de re consent to de re deception. If this is right, rather than saying, as Sommers does, that deception generally fails to invalidate commonsense consent, we should refine our understanding of commonsense consent to include two varieties of consent, each of which is in fact invalidated by its own variety of deception.

Responding to recent concerns about the reliability of the published literature in psychology and other disciplines, we formed the X-Phi Replicability Project to estimate the reproducibility of experimental philosophy. Drawing on a representative sample of 40 x-phi studies published between 2003 and 2015, we enlisted 20 research teams across 8 countries to conduct a high-quality replication of each study in order to compare the results to the original published findings. We found that x-phi studies – as represented in our sample – successfully replicated about 70% of the time. We discuss possible reasons for this relatively high replication rate in the field of experimental philosophy and offer suggestions for best research practices going forward.

In his 2005 paper, DeClercq observes that aesthetic terms such as ‘beautiful’, ‘elegant’, ‘harmonious’, etc. resist metaphorical interpreta­tion and argues that it is the fact that such terms cannot be involved in category-mistakes that explains their metaphorical uninterpretability. While I largely agree with DeClercq’s observation of the metaphorical uninterpret­ability of aesthetic terms, I offer both non-empirical and empirical considerations against his category-based explanation of the phenomenon. I offer the former in a longer version of this paper. In this shorter version, I offer preliminary, empirical evidence against DeClercq’s thesis that the possibility of category mistakes is what is required for metaphor judgments. I do this, in part, by relying on the work of cultural psychologist, Nisbett, who found that, in general, Easterners rely less on categories and more on relational and contextual information than Westerners in making judgments and reasoning inferentially. I report the results of my cross-cultural study on metaphor, which cast some doubt on DeClercq’s thesis that it is the possibility of category-mistakes that explain the metaphorical interpretability of expressions in general.

A general feature of language that appears to resist systematic semantic analysis is context-sensitivity. Since the birth of analytic philosophy, philosophers have thought the context-dependence of natural language renders it unsuitable for analysis by the semantic tools of the logician. And metaphor appears to pose a particularly vexing problem in that, in addition to being difficult to systematize for other reasons, it is also context-dependent. However in recent years, the problem of context-dependency has moved to the foreground in the philosophy of language. And some theorists have taken on the daunting challenge of accounting forour context-variant intuitions about what is said by systematic means. The central point of this paper then is to show that the resources these theorists use are far more powerful than the theorists realize. I argue that if these theorists are correct about context-sensitivity, the same resources they use to make context-sensitivity compatible with semantic systematicity can be used to yield a systematic semantic account of metaphor. The paper can be viewed as either a powerful consideration against adopting the resources of theorists who seek to explain all contextsensitivity semantically, or a powerful consideration against those whobelieve metaphor to be merely a matter of language use.

In this article, I start with the observation that aesthetic terms resist metaphorical interpretation; that is, it makes little sense to say that something is beautiful metaphorically speaking or to say something is metaphorically elegant, harmonious, or sublime. I argue that aesthetic terms’ lack of metaphorical interpretations is not explained by the fact that their applicability is not limited to a particular category of objects, at least in the standard sense of ‘category.’ In general, I challenge category-based accounts of metaphorical interpretability and instead offer an alternative explanation for aesthetic terms’ lack of metaphorical interpretations, one that involves the notion of context shifts rather than category violations. I argue that what is required for metaphorical interpretability is the joint satisfaction of two conditions: multidimensionality and the presence of a default dimension. Aesthetic terms lack metaphorical readings because they fail to satisfy, even though they satisfy. I argue that the alternative account I offer is predictively adequate, more parsimonious, less subject to counterexamples, and hence preferable to the category-based one

The purpose of this study is to investigate the relationships among corporate social responsibility (CSR), corporate brand credibility, corporate brand equity, and corporate reputation. Structural equation modeling analysis provided support for the hypotheses from a sample of 867 consumers in South Korea. The results showed that CSR has a direct positive effect on corporate brand credibility and corporate reputation. In addition, the results indicate that corporate brand credibility mediates the relationship between CSR and corporate reputation. Moreover, corporate brand credibility mediates the relationship between CSR and corporate reputation. Finally, the relationship between CSR and corporate brand equity is sequentially and fully mediated by corporate brand credibility and corporate reputation. The theoretical and managerial implications of the results and limitations are discussed, and future research directions are suggested