Qiuqi Li

© 2016, American Society for Biochemistry and Molecular Biology Inc. All rights reserved.Glycyl-tRNA synthetase is the enzyme that covalently links glycine to cognate tRNA for translation. It is of great research interest because of its nonconserved quaternary structures, unique species-specific aminoacylation properties, and noncanonical functions in neurological diseases, but none of these is fully understood. We report two crystal structures of human GlyRS variants, in the free form and in complex with tRNAGly respectively, and reveal new aspects of the glycylation mechanism. We discover that insertion 3 differs considerably in conformation in catalysis and that it acts like a "switch" and fully opens to allow tRNA to bind in a cross-subunit fashion. The flexibility of the protein is supported by molecular dynamics simulation, as well as enzymatic activity assays. The biophysical and biochemical studies suggest that human GlyRS may utilize its flexibility for both the traditional function and alternative functions.

In the discussion whether Confucianism supports human rights, it is necessary to distinguish between the content and the form of human rights. Regarding the content of human rights, only the normative texts in Confucianism can contribute to the discussion. Even though Confucianism concedes that people are equal in nature, this equality is restricted in certain areas of normative justification. Regarding the form of human rights, the Confucian idea of graded love is against the universal nature of human rights. However, the pre-ontology of Heidegger shows us how graded love can actually be the ground of the Confucian approach to universality, which overcomes the opposition between universality and particularity within Western metaphysics. Therefore, in the Confucian approach to universality, graded love can be the motivation to universalizing of human rights. Moreover, as the Confucian approach to universality is not a principle that transcends all particularities, it helps construct an inclusive form of human rights.