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    Endoribonuclease-Based Two-Component Repressor Systems for Tight Gene Expression Control in Plants
    with Y. Liang, J. Yan, V. T. Benites, C. Cheng-Yue, T. Tran, J. Mortimer, A. Mukhopadhyay, J. D. Keasling, H. V. Scheller, and D. Loqué
    Tight control and multifactorial regulation of gene expression are important challenges in genetic engineering and are critical for the development of regulatory circuits. Meeting these challenges will facilitate transgene expression regulation and support the fine-tuning of metabolic pathways to avoid the accumulation of undesired intermediates. By employing the endoribonuclease Csy4 and its recognition sequence from Pseudomonas aeruginosa and manipulating 5'UTR of mRNA, we developed a two-comp…Read more