Tara Anderson

In addition to chronological age, humans perceive themselves and others as possessing several different functional ages. We extended the boundaries of research on perceived age concepts to cats and dogs, asking people to report on the best physical, mental, emotional, and social ages for cats and dogs. We found that emotional age was the oldest of the best ages and that physical best age was the youngest perceived best age for humans, cats, and dogs. Subjective age concepts extend across species to cats and dogs, and humans’ subjective self age estimates correlate with their estimates of nonhuman animal subjective ages. Human estimates of animal ages may influence variables such as adoption and animal care.

Improved tools for providing specific intraoperative diagnoses could improve patient care. In neurosurgery, intraoperatively differentiating non-operative lesions such as CNS B-cell lymphoma from operative lesions can be challenging, often necessitating immunohistochemical procedures which require up to 24-48 hours. Here, we evaluate the feasibility of generating rapid ex vivo specific labeling using a novel lymphoma-specific fluorescent switchable aptamer. Our B-cell lymphoma-specific switchable aptamer produced only low-level fluorescence in its unbound conformation and generated an 8-fold increase in fluorescence once bound to its target on CD20-positive lymphoma cells. The aptamer demonstrated strong binding to B-cell lymphoma cells within 15 minutes of incubation as observed by flow cytometry. We applied the switchable aptamer to ex vivo xenograft tissue harboring B-cell lymphoma and astrocytoma, and within one hour specific visual identification of lymphoma was routinely possible. In this proof-of-concept study in human cell culture and orthotopic xenografts, we conclude that a fluorescent switchable aptamer can provide rapid and specific labeling of B-cell lymphoma, and that developing aptamer-based labeling approaches could simplify tissue staining and drastically reduce time to histopathological diagnoses compared with IHC-based methods. We propose that switchable aptamers could enhance expeditious, accurate intraoperative decision-making.

The purpose of this study is to extend and replicate previously published results from a random probability sample of university faculty. The sample assessed reasons given for companion-animal guardianship and for belief in the beneficial health effects of owning pets. In this replication and extension design, these two non-random samples responded to the same questionnaire items as those addressed to university faculty. Results indicated that avoidance of loneliness was the most frequent reason for owning pets among both students and middle-aged community members. This result is consistent with the view that animals provide social support and companionship to humans at various stages of the life cycle. Suggesting an emergent feature of the human-nonhuman animal bond, both groups selected, “the pet helps keep me active” as the second-most common reason. Older women reported a greater belief in the health-giving benefits of pet ownership than did younger persons or men. This belief may contribute to human benefits of pet ownership and requires further research