Yong Liu

Copyright © 2015 by ASME.This paper replaces the hinged pivots of an eight-bar linkage with flexure joints in order to achieve a flexure-connected linkage system that guides rectilinear movement of its end-effector. The goal is a linkage design that can be reduced in size to provide a suspension for the proof masses of a MEMS gyroscope. The symmetric design of the linkage and its long travel relative to other MEMS suspensions has the potential to provide a number of advantages, such as the reduction of quadrature error. The design presented yields 0.1% deviation over its range of movement. An example also presents the driving linkage of the MEMS gyroscope, which is also designed as flexure connected linkage.

© 2015 by The American Society for Biochemistry and Molecular Biology Inc. Plasminogen activator inhibitor 1 level is extremely elevated in the edematous fluid of acutely injured lungs and pleurae. Elevated PAI-1 specifically inactivates pulmonary urokinase-type and tissue-type plasminogen activators. We hypothesized that plasminogen activation and fibrinolysis may alter epithelial sodium channel activity, a key player in clearing edematous fluid. Two-chain urokinase has been found to strongly stimulate heterologous human αβγ ENaC activity in a dose- and time-dependent manner. This activity of tcuPA was completely ablated by PAI-1. Furthermore, a mutation of the active site of the enzyme also prevented ENaC activation. By comparison, three truncation mutants of the amino-terminal fragment of tcuPA still activated ENaC. uPA enzymatic activity was positively correlated with ENaC current amplitude prior to reaching the maximal level. In sharp contrast to uPA, neither single-chain tPA nor derivatives, including two-chain tPA and tenecteplase, affected ENaC activity. Furthermore, γ but not α subunit of ENaC was proteolytically cleaved at by tcuPA. In summary, the underlying mechanisms of urokinase-mediated activation of ENaC include release of self-inhibition, proteolysis of γ ENaC, incremental increase in opening rate, and activation of closed channels. This study for the first time demonstrates multifaceted mechanisms for uPA-mediated up-regulation of ENaC, which form the cellular and molecular rationale for the beneficial effects of urokinase in mitigating mortal pulmonary edema and pleural effusions.

Background: Anopheles sinensis is a major malaria vector in China and other Southeast Asian countries, and it is becoming increasingly resistant to the insecticides used for agriculture, net impregnation, and indoor residual spray. Very limited genomic information on this species is available, which has hindered the development of new tools for resistance surveillance and vector control. We used the 454 GS FLX system and generated expressed sequence tag databases of various life stages of An. sinensis, and we determined the transcriptional differences between deltamethrin resistant and susceptible mosquitoes.Results: The 454 GS FLX transcriptome sequencing yielded a total of 624,559 reads with the pooled An. sinensis mosquitoes across various development stages. The de novo assembly generated 33,411 contigs with average length of 493 bp. A total of 8,057 ESTs were generated with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes annotation. A total of 2,131 ESTs were differentially expressed between deltamethrin resistant and susceptible mosquitoes collected from the same field site in Jiangsu, China. Among these differentially expressed ESTs, a total of 294 pathways were mapped to the KEGG database, with the predominant ESTs belonging to metabolic pathways. Furthermore, a total of 2,408 microsatellites and 15,496 single nucleotide polymorphisms were identified.Conclusions: The annotated EST and transcriptome databases provide a valuable genomic resource for further genetic studies of this important malaria vector species. The differentially expressed ESTs associated with insecticide resistance identified in this study lay an important foundation for further functional analysis. The identified microsatellite and SNP markers will provide useful tools for future population genetic and comparative genomic analyses of malaria vectors. © 2014 Zhu et al.; licensee BioMed Central Ltd.

© 2014 Li et al. Aedes albopictus is a very invasive and aggressive insect vector that causes outbreaks of dengue fever, chikungunya disease, and yellow fever in many countries. Vector ecology and disease epidemiology are strongly affected by environmental changes. Urbanization is a worldwide trend and is one of the most ecologically modifying phenomena. The purpose of this study is to determine how environmental changes due to urbanization affect the ecology of Aedes albopictus. Aquatic habitats and Aedes albopictus larval population surveys were conducted from May to November 2013 in three areas representing rural, suburban, and urban settings in Guangzhou, China. Ae. albopictus adults were collected monthly using BG-Sentinel traps. Ae. albopictus larva and adult life-table experiments were conducted with 20 replicates in each of the three study areas. The urban area had the highest and the rural area had the lowest number of aquatic habitats that tested positive for Ae. albopictus larvae. Densities in the larval stages varied among the areas, but the urban area had almost two-fold higher densities in pupae and three-fold higher in adult populations compared with the suburban and rural areas. Larvae developed faster and the adult emergence rate was higher in the urban area than in suburban and rural areas. The survival time of adult mosquitoes was also longer in the urban area than it was in suburban and rural areas. Study regions, surface area, water depth, water clearance, surface type, and canopy coverage were important factors associated with the presence of Ae. albopictus larvae. Urbanization substantially increased the density, larval development rate, and adult survival time of Ae. albopictus, which in turn potentially increased the vector capacity, and therefore, disease transmissibility. Mosquito ecology and its correlation with dengue virus transmission should be compared in different environmental settings.

Ben shu yi Ming ru Li Cai de jiang xue huo dong yu xue pai jian gou wei zhong xin, tan tao cong shi liu shi ji chu dao shi qi shi ji mo li xue qun ti zhong xin xing xue shuo he xue pai de chan sheng, chuan bo, kuo zhan yu chuan cheng de mo shi, bing zhuo zhong cong si xiang shi de jiao du jie shi zhe zhong mo shi chan sheng he yun xing de si xiang gen yuan yu nei zai dong li. Quan shu zheng wen gong qi zhang, zhu yao nei rong fen wei shang xia liang bian, shang bian yi hong guan zong lun de fang shi, cong li xue jing dian "Da xue" de wen ben bian dong he quan shi jing zheng qie ru, tan tao zhong wan Ming li xue xue shuo he xue pai jian gou de li lun gen ju; xia bian yi Li Cai de "zhi xiu" xue shuo ji qi jiang xue huo dong zuo wei ge an yan jiu, tan tao li xue xue shuo he xue pai de chuan bo ji chuan cheng de she hui hua tu jing.

This dissertation is highly motivated by d.r.e. Nondensity Theorem, which is interesting in two perspectives. One is that it contrasts Sacks Density Theorem, and hence shows that the structures of r.e. degrees and d.r.e. degrees are different. The other is to investigate what other properties a maximal degree can have.In Chapter 1, we briefly review the backgrounds of Recursion Theory which motivate the topics of this dissertation.In Chapter 2, we introduce the notion of $$ -cupping degree. It is closely related to the notion of maximal d.r.e. degree. In fact, a $$ -cupping degree is maximal d.r.e. degree. We then prove that there exists an isolated $$ -cupping degree by combining strategies for maximality and isolation with some efforts.Chapter 3 is part of a joint project with Steffen Lempp, Yiqun Liu, Keng Meng Ng, Cheng Peng, and Guohua Wu. In this chapter, we prove that any finite boolean algebra can be embedded into d.r.e. degrees as a final segment. We examine the proof of d.r.e. Nondensity Theorem and make developments to the technique to make it work for our theorem. The goal of the project is to see what lattice can be embedded into d.r.e. degrees as a final segment, as we observe that the technique has potential be developed further to produce other interesting results.prepared by Yong Liu.E-mail: [email protected].

Demographic characteristics have been recognized as an important factor affecting public acceptance of waste-to-energy incineration facilities. The present study explores whether the differences in public acceptance of WTE incineration facilities caused by demographic characteristics are consistent in residential groups under different perceived stress using data collected by a large-scale questionnaire survey conducted in three second-tier cities in China. The result of data analysis using a T-test shows firstly that people with low perceived stress have higher public acceptance of WTE incineration facilities. Second, the differences in public acceptance of WTE incineration facilities caused by demographic characteristics vary in residential groups with different perceived stress levels. The findings enrich the knowledge system related to demographic characteristics research on NIMBY infrastructure projects and provide the theoretical basis for the government to formulate more targeted policies about NIMBY infrastructure sitting.

People have three cognitive representations of the self, namely, the individual, relational, and collective selves, which are indispensable components of selfhood but not necessarily given equal preference. Previous studies found that people displayed varied self-hierarchy in miscellaneous tasks involving different research materials that had pre-existing learned associations established over long periods of time. Therefore, this study tries to explore a purer self-hierarchy without the influence of research materials, using perceptual matching tasks. The behavioral and event-related potentials’ findings showed that people recognized information association with their individual self faster compared with their relational and CS. Smaller N2, stronger P3 and late positive complex amplitudes were evoked during IS compared with RS and CS. However, the three selves evoked equal P2 amplitudes at the early processing stage. Moreover, CS showed a weaker advantage than RS, demonstrating a longer reaction time, lower d prime, and weaker P3 and LPC amplitudes in the parietal region. Overall, self-hierarchy during simple perceptual processing manifested as IS > RS > CS at the late processing stage but manifested as IS = RS = CS at the early processing stage. Self-hierarchy varies according to the processing stage, even without meaningful information and during the simple perception processing. This result provides direct evidence that all selves can be tagged with neutral actions, which would fit the idea of an organism attuned to self-survival at multiple processing levels.

Physical literacy has received considerable attention in the field of physical education and physical activity worldwide. According to recent studies, the level of physical fitness among Chinese university students is gradually decreasing. This study aims to examine the impact of the PF level on PA and PL, as well as the relationships among PF, PA, and PL, in Chinese university students. Participants comprised 798 university students in Chongqing, China. Participants completed the tests of vital capacity, cardiorespiratory fitness, muscular strength, and flexibility, according to the National Physical Fitness Measurement Standards Manual, as well as questionnaires on PA and PL. The independent t-tests were conducted to examine sex differences in the evaluated variables, and the Pearson’s correlation between each PF test and PL attributes and PA was calculated according to sex. In addition, the independent t-tests were conducted to determine whether the PF level had an effect on PL attributes and PA at various intensities. Significant sex differences were found in the PF domains of vital capacity, muscular strength, and aerobic fitness, with higher scores in men than in women, but not in the PF domain of flexibility and total PL score. Furthermore, the PF domains of muscular strength and aerobic fitness were significantly and positively correlated with the PL attributes of confidence and physical competence in both men and women, while the PF domains of vital capacity and aerobic fitness were significantly and positively correlated with the PL attribute of motivation in men. In addition, PL was significantly and positively associated with cardiorespiratory fitness, vital capacity, muscular strength, and flexibility among participants in the fit group. These findings support advocating for increased participation in PA in university students and using PL as a tool to improve PF components.