Zhe Wang

Trend and change-point analyses of water quality time series data have important implications for pollution control and environmental decision-making. This paper developed a new approach to assess trends and change-points of water quality parameters by integrating locally weighted polynomial regression and segmented regression. Firstly, LWPR was used to pretreat the original water quality data into a smoothed time series to represent the long-term trend of water quality. Then, SegReg was used to identify the long-term trends and change-points of the smoothed time series. Finally, statistical tests were applied to determine the significance of the long-term trends and change-points. The efficacy of this approach was validated using a 10-year record of total nitrogen and chemical oxygen demand from Shanxi Reservoir watershed in eastern China. Results showed that this approach was straightforward and reliable for assessment of long-term trends and change-points on irregular water quality datasets. The reliability was verified by statistical tests and practical considerations for Shanxi Reservoir watershed. The newly developed integrated LWPR-SegReg approach is not only limited to the assessment of trends and change-points of water quality parameters but also has a broad application to other fields with long-term time series records.

Electron cryomicroscopy has been used to determine the atomic coordinates from density maps of biological assemblies. These models can be assessed by their overall fit to the experimental data and stereochemical information. However, these models do not annotate the actual density values of the atoms nor their positional uncertainty. Here, we introduce a computational procedure to derive an atomic model from a cryo- EM map with annotated metadata. The accuracy of such a model is validated by a faithful replication of the experimental cryo-EM map computed using the coordinates and associated metadata. The functional interpretation of any structural features in the model and its utilization for future studies can be made in the context of its measure of uncertainty. We applied this protocol to the 3.3-Å map of the mature P22 bacteriophage capsid, a large and complex macromolecular assembly.With this protocol, we identify and annotate previously undescribed molecular interactions between capsid subunits that are crucial to maintain stability in the absence of cementing proteins or cross-linking, as occur in other bacteriophages.

Plasmon-generated hot carriers are used in photovoltaic or photochemical applications. However, the interplays between the plasmon and single-particle excitations in nanosystems have not been theoretically addressed using ab initio methods. Here we show such interplays in a Ag 55 nanocluster using real-time time-dependent density functional theory simulations. We find that the disappearance of the zero-frequency peak in the Fourier transform of the band-to-band transition coefficient is a hallmark of the plasmon. We show the importance of the d-states for hot-carrier generations. If the single-particle d-to-s excitations are resonant to the plasmon frequency, the majority of the plasmon energy will be converted into hot carriers, and the overall hot-carrier generation is enhanced by the plasmon; if such resonance does not exist, we observe an intriguing Rabi oscillation between the plasmon and hot carriers. Phonons play a minor role in plasmonic dynamics in such small systems. This study provides guidance on improving plasmonic applications.

© 2016, American Society for Biochemistry and Molecular Biology Inc. All rights reserved.Glycyl-tRNA synthetase is the enzyme that covalently links glycine to cognate tRNA for translation. It is of great research interest because of its nonconserved quaternary structures, unique species-specific aminoacylation properties, and noncanonical functions in neurological diseases, but none of these is fully understood. We report two crystal structures of human GlyRS variants, in the free form and in complex with tRNAGly respectively, and reveal new aspects of the glycylation mechanism. We discover that insertion 3 differs considerably in conformation in catalysis and that it acts like a "switch" and fully opens to allow tRNA to bind in a cross-subunit fashion. The flexibility of the protein is supported by molecular dynamics simulation, as well as enzymatic activity assays. The biophysical and biochemical studies suggest that human GlyRS may utilize its flexibility for both the traditional function and alternative functions.

© 2014 Nature America, Inc. All rights reserved. Using genome-wide data from 253,288 individuals, we identified 697 variants at genome-wide significance that together explained one-fifth of the heritability for adult height. By testing different numbers of variants in independent studies, we show that the most strongly associated 1/42,000, 1/43,700 and 1/49,500 SNPs explained 1/421%, 1/424% and 1/429% of phenotypic variance. Furthermore, all common variants together captured 60% of heritability. The 697 variants clustered in 423 loci were enriched for genes, pathways and tissue types known to be involved in growth and together implicated genes and pathways not highlighted in earlier efforts, such as signaling by fibroblast growth factors, WNT/I 2-catenin and chondroitin sulfate-related genes. We identified several genes and pathways not previously connected with human skeletal growth, including mTOR, osteoglycin and binding of hyaluronic acid. Our results indicate a genetic architecture for human height that is characterized by a very large but finite number of causal variants.

With their focus on human production and consumption activities, cities incur massive energy consumption and CO2 emissions. An intercity connection is a typical complex system in which the interaction between cities is crucial for developing low-carbon outputs within the urban agglomeration. This paper presents the construction of the CO2 emission network of an urban agglomeration in the Yangtze River middle reaches megalopolis, based on the gravity model. Combined with social network analysis, a multilevel analysis framework is proposed to deal with the complexity, spatiality, and visualization of the CO2 emission network with reference to the network features, structural equivalence, and the rich-club phenomenon. The following results emerged: firstly, the spatial structure of the CO2 emissions was characterized by low robustness and compactness, indicating disunity among the studied cities. Secondly, there was found to be a strong correlation between regionalism and intercity connections, with geographically close cities playing a similar role in the network. Thirdly, the “rich-club” cities, including Wuhan, Changsha, Xiaogan, and Zhuzhou, dominated the connections, covering more than 87.1% of the network in the Yangtze River Middle Reaches Megalopolis.

Previous studies suggested that facial attractiveness perception can be increased with facial skin homogeneity improving; and human’s facial change detection increases along with facial skin homogeneity increases. However, it’s unknown whether a face can be perceived prettier than it did before while still being considered as physically the same. It is possible that these two kinds of cognitive-aesthetic processing may have separate mathematical functions in psychophysical studies. In other words, human’s facial attractiveness differentiation may be more sensitive than facial change detection. In this current study, we explored the above questions. Using three types of psychophysical techniques to manipulate facial skin homogeneity, we measured how participants’ sensitivity to facial skin homogeneity and attractiveness change. Results showed a linear function curve for facial physical change detection and a logarithmic function curve was drawn in the forced-choice technique, which was the most sensitive one, indicating that participants can judge a face prettier than before without being aware of it has physically changed. Besides, two linear function curves were shown in the same/different technique and a rating technique. Taken together, this current study revealed that facial attractiveness can be enhanced and discriminated by improving facial skin homogeneity, without being realized by people with conscious awareness that the face has been changed.

Previous studies have shown that social categorization can induce an own-group face recognition bias. However, similar and better other-group face recognition emerged recently. In this research, we aimed to examine whether competitive cues and group status accompanied by social categorization can modulate the inter-group face recognition bias. Moreover, we investigated how the group identification of individuals with different statuses affected the inter-group face recognition bias. The results indicated that an own-group face recognition bias emerged for targets with in-group labels compared to out-group labels. Moreover, when the group labels signaled competitive cues, the own-group face recognition bias was reversed. Furthermore, low-status and similar-status individuals exhibited out-group face recognition bias, but high-status individuals did not. In addition, the higher the in-group identification scores of participants from the low-status group, the stronger the out-group face recognition bias. These results suggested that competitive cues would reverse the own-group face recognition bias and the group status would play a modulating role in face recognition bias.

This study investigated the longer-term impacts of trained peer feedback in comparison with teacher feedback on students’ writing development and writing motivation. Sections of an EFL writing course were randomly assigned to either teacher feedback or trained peer feedback conditions across two semesters. In the first semester, during their writing class, students either received training in how to implement peer feedback or simply studied models of writing. In the second semester, students either received teacher or peer feedback across multiple assignments. Writing competence, writing self-efficacy, and writing self-regulated learning were assessed at the beginning and end of the second semester. Trained peer feedback and teacher feedback had similar positive effects on the improvement of writing competence and writing self-efficacy. However, trained peer feedback led to a significant enhancement of students’ autonomous motivation relative to no such growth from teacher feedback.

The regulatory effect of embodied emotion on one’s general emotion and the impact of the compatibility or incompatibility of the two types of emotion on creative thinking are still debatable. The purpose of this study is to investigate these issues experimentally. In Experiment 1, participants completed an explicit positive and negative emotion test [Positive and Negative Affect Schedule (PANAS)] and an implicit positive and negative emotion test [Implicit Positive and Negative Affect Test (IPANAT)] twice on a computer after emotional video priming was used to induce negative emotions and facial expression manipulation was performed to induce embodied positive or negative emotions. It was found that maintaining the expression of a suppressed smile was helpful in regulating negative emotions ( p = 0.047). Specifically, the implicit negative emotions induced by facial expression manipulation had a positive regulating effect on the implicit negative emotions induced by the video (T1, M = 47.813; to T2, M = 44.188). In Experiment 2, the positive or negative emotions of the participants were induced using emotional videos, and facial expression manipulation was used to induce their embodied positive or negative emotions. Then, the participants completed a creative test by completing alternative use tasks (AUTs) and Chinese character riddles. The AUT fluency score in the emotionally compatible group was significantly higher than that in the emotionally incompatible group ( p = 0.032), but while experiencing negative emotions, the emotionally compatible group had a significantly higher originality score and insight in Chinese character riddle score than the emotionally incompatible group ( p = 0.017, p = 0.004). Therefore, embodied negative emotion has a significant regulating effect on implicit negative emotion. The compatibility of emotion activated by facial expression and viewing a video contributes to creative thinking, whereas the incompatibility of emotion hinders creative thinking. The compatibility of emotion under positive emotions improved thinking fluency, whereas under negative emotions, it activated originality and insight in creative thinking. The influence of such emotional compatibility on creative thinking may be due to the regulating effect of embodied emotions on implicit emotions induced by emotional stimuli.

Multiphoton-targeted photochemistry was used to selectively inactivate the expression of genes in vertebrate cells. A membrane permeable DNA-associating vital dye, ethidium bromide monoacetate was used to photosensitize chromosomes in dividing cells. A 100-ps infrared laser beam operating at 1.06 microns was focused onto a selected region of a mitotic chromosome corresponding to the sites of the nucleolar genes. Individual cells followed through mitosis demonstrated a reduction in the number of nucleoli formed in daughter cells that corresponded to the number of nucleolar genes sites irradiated. These results demonstrate the ability to selectively manipulate genes by using the focal point specificity characteristic of multiphoton microscopy. This technique should have wide biotechnology applications both in vitro and in vivo.

A fundamental way in which human thought has developed has been constantly to explain the earliest "classics" that are the source of that thought. All in all, the number of such classics is not very high, their explanations are past counting. Moreover, they are constantly increasing, giving rise to an explanatory chain deriving from the classics. In the development of Chinese philosophy, this aspect is particularly noticeable, so that one can describe Chinese philosophy as a continual explanation of the classics. This holds for both Confucianism and Daoism. The main classics of Daoism are the Laozi and the Zhuangzi. These two works have been constantly reread and reinterpreted throughout history. From the late nineteenth century onward, Chinese philosophy came into closer contact with Western philosophy. Foreign concepts were brought in to provide philosophers with new "insight." Some thinkers applied this new insight or these foreign concepts to the Daoist classics. In this way, they brought a new explanation of the Daoist classics and enriched the ways of interpreting the texts.1 Paving the way in this direction were Yan Fu (1853-1921). Zhang Taiyan (1869-1936), Liang Qichao (1873-1929), Wang Guowei (1877-1927), and Hu Shi (1891-1962).

This will be the traditional theory of natural law jurisprudence of Thomas Aquinas in the most representative point of view, examining the legal representative of the modern pure raw ─ ─ Kyle's criticism of natural law theory. Is extolled its purely legal claims, Kyle Western Health had made ​​various theories of natural law and a wide range of strong criticism, this paper will briefly present their criticism. Next, this article will be Thomas Aquinas legal point of view, examining the questions of the Kyle of Health proposed is sufficient to refute the theory of natural law. This article will explain, Kyle theoretical basis of health or their own is questionable, or their misunderstanding of natural law theory to the critique of natural law jurisprudence of Thomas Aquinas at least, the rate is more than can not be established