Yaming Li

© 2016 American Society of Plant Biologists. All rights reserved.Calcium/calmodulin-dependent protein kinase has been shown to play an important role in abscisic acid -induced antioxidant defense and enhance the tolerance of plants to drought stress. However, its downstream molecular events are poorly understood. Here, we identify a NAC transcription factor, ZmNAC84, in maize, which physically interacts with ZmCCaMK in vitro and in vivo. ZmNAC84 displays a partially overlapping expression pattern with ZmCCaMK after ABA treatment, and H2O2 is required for ABA-induced ZmNAC84expression. Functional analysis reveals that ZmNAC84 is essential for ABA-induced antioxidant defense in a ZmCCaMK-dependent manner. Furthermore, ZmCCaMK directly phosphorylates Ser-113 of ZmNAC84 in vitro, and Ser-113 is essential for the ABA-induced stimulation of antioxidant defense by ZmCCaMK. Moreover, overexpression of ZmNAC84 in tobacco can improve drought tolerance and alleviate drought-induced oxidative damage of transgenic plants. These results define a mechanism for ZmCCaMK function in ABA-induced antioxidant defense, where ABA-produced H2O2 first induces expression of ZmCCaMK and ZmNAC84 and activates ZmCCaMK. Subsequently, the activated ZmCCaMK phosphorylates ZmNAC84 at Ser-113, thereby inducing antioxidant defense by activating downstream genes.

© 2014 Elsevier B.V.. Nitrogen pollution is a global environmental problem that has greatly increased the risks of both the eutrophication of surface waters and contamination of ground waters. The majority of N pollution mainly comes from agricultural fields, in particular during rice growing seasons. In recent years, a gradual shift from the transplanting rice cultivation method to the direct seeding method has occurred, which results in different water and N losses from paddy fields and leads to distinct impacts on water environments. The N transport and transformations in an experimental direct-seeded-rice field in the Taihu Lake Basin of east China were observed during two consecutive seasons, and simulated using Hydrus-1D model. The observed crop N uptake, ammonia volatilization, N concentrations in soil, and N leaching were used to calibrate and validate the model parameters. The two most important inputs of N, i.e., fertilization and mineralization, were considered in the simulations with 220 and 145.5kgha-1 in 2008 and 220 and 147.8kgha-1 in 2009, respectively. Ammonia volatilization and nitrate denitrification were the two dominant pathways of N loss, accounting for about 16.0% and 38.8% of the total N input, respectively. Both nitrification and denitrification processes mainly occurred in the root zone. N leaching at 60 and 120cm depths accounted for about 6.8% and 2.7% of TNI, respectively. The crop N uptake was 32.1% and 30.8% of TNI during the 2008 and 2009 seasons, respectively, and ammonium was the predominant form. Simulated N concentrations and fluxes in soil matched well with the corresponding observed data. Hydrus-1D could simulate the N transport and transformations in the DSR field, and could thus be a good tool for designing optimal fertilizer management practices in the future.

In the recent decade, increasing costs of labor, water, and fertilizers around the world led to a change in the method of crop establishment from traditional transplanted rice to direct-seeded rice and to a substantial rise in the DSR-managed area. Since water management in areas with DSR is quite different from those with TPR, vertical water movement and water and nutrient losses during the crop season may be different as well. Water flow and water losses in a DSR field in the Taihu Lake Basin of east China were monitored and evaluated using Hydrus-1D during two seasons with different rainfalls and irrigation managements. While during the 2008 season, irrigation accounted for 57% of the total water input, during the 2009 season, it accounted for only 32%. Due to large rainfall during the wet, 2009 rice season, surface runoff accounted for about 17.0% of TWI. During the much drier 2008 rice season with higher irrigation inputs, surface runoff could be controlled much better. Modeled evapotranspiration during the 2008 and 2009 seasons accounted for 54.6% and 44.6% of TWIs, respectively. Measured and simulated results indicate that water leaching was the main path of water loss from the DSR fields, which implies that frequent irrigation increases water leaching. The plough sole layer played a major buffering role for water flow during both dry and wet seasons. Water productivities evaluated from TWIs during the 2008 and 2009 seasons were 0.71 and 0.59kg/m3, respectively; they were 1.30 and 1.33kg/m3 when evaluated from modeled evapotranspiration fluxes. Pressure heads and vertical fluxes simulated using Hydrus-1D matched measured data well. The Hydrus-1D can be used to simulate water flow and water balance in the DSR fields. © 2014 Elsevier B.V.

© 2014 Li et al. Aedes albopictus is a very invasive and aggressive insect vector that causes outbreaks of dengue fever, chikungunya disease, and yellow fever in many countries. Vector ecology and disease epidemiology are strongly affected by environmental changes. Urbanization is a worldwide trend and is one of the most ecologically modifying phenomena. The purpose of this study is to determine how environmental changes due to urbanization affect the ecology of Aedes albopictus. Aquatic habitats and Aedes albopictus larval population surveys were conducted from May to November 2013 in three areas representing rural, suburban, and urban settings in Guangzhou, China. Ae. albopictus adults were collected monthly using BG-Sentinel traps. Ae. albopictus larva and adult life-table experiments were conducted with 20 replicates in each of the three study areas. The urban area had the highest and the rural area had the lowest number of aquatic habitats that tested positive for Ae. albopictus larvae. Densities in the larval stages varied among the areas, but the urban area had almost two-fold higher densities in pupae and three-fold higher in adult populations compared with the suburban and rural areas. Larvae developed faster and the adult emergence rate was higher in the urban area than in suburban and rural areas. The survival time of adult mosquitoes was also longer in the urban area than it was in suburban and rural areas. Study regions, surface area, water depth, water clearance, surface type, and canopy coverage were important factors associated with the presence of Ae. albopictus larvae. Urbanization substantially increased the density, larval development rate, and adult survival time of Ae. albopictus, which in turn potentially increased the vector capacity, and therefore, disease transmissibility. Mosquito ecology and its correlation with dengue virus transmission should be compared in different environmental settings.

Head transplantation has ignited intense discussions about whether it should be done scientifically and ethically. This paper examines the ethics of head transplantation from a Confucian perspective and offers arguments against the permissibility of head transplantation. From a Confucian point of view, human beings are the most precious organisms in the world, and ren and li are the basic moral principles of human beings. As long as head transplant technology remains underdeveloped, this procedure should not be done because it will pose a serious risk of harm to humans and thus violate the principles of Confucian ren and li. If head transplant technology matures to the point that it would be safe to try in humans, it still should not be performed because it would change the selfhood or identity of the head donor and the body donor and create a new person. Confucian personal virtues greatly depend on selfhood or personal identity, and selfhood or personal identity depend on one’s body cultivation and mind rectification. Through the hard effort of body cultivation and mind rectification, one transforms one’s selfhood or identity and personality. This selfhood, identity, and personality cannot be separated from one’s body but are embedded in one’s body. Thus, head transplant would destroy two persons’ identities and result in a new person, and the characteristics of this new person’s identity are still unknown.

Human dignity is a crucial concept in contemporary ethical, political and legal studies. However, people have different, even opposite understandings of human dignity, which has caused lots of confusion in related discourses. The Confucian notion of human dignity provides an important perspective for reflecting various theories of human dignity. In Confucian ethics, the basis of human dignity is the moral potential that every human being naturally has. Moral potential grants everyone universal dignity, while the development of moral potential grants people acquired dignity. Since all human beings have moral potential to the same extent, everyone owns universal dignity equally. Different people develop their moral potential to different extents. One’s acquired dignity is positively associated with the development of her moral potential. Universal dignity is a moral status but acquired dignity is not. To pursue acquired dignity is the moral demand of universal dignity and protects people’s universal dignity. Confucian discussions on human dignity provide not only a way to justify the equality of human moral status, but also a way to justify the moral obligation of protecting and developing human nature, thereby constituting a reliable theoretical basis for coping with ethical issues caused by biomedical technologies today.

Death with dignity is a significant issue in modern bioethics. In modern healthcare, the wide use of new technologies at the end of life has caused heated debate on how to protect human dignity. The key point of contention lies in the different understandings of human dignity and the dignity of death. Human dignity has never been a clear concept in Western ethical explorations, and the dignity of death has given rise to more confusions. Although there is no such term as “dignity” in Confucian ethics, there are discussions of a number of ideas related to human dignity and the dignity of death. Therefore, Confucian bioethics can offer a new perspective for understanding the theoretical difficulties associated with the dignity of death and new methods for solving them. In this article, we attempt to reconstruct Confucian views on human dignity and the dignity of death and, based on those views, to analyze the following issues: the relationship between the dignity of death and biological life, the relationship between the dignity of death and suffering, the relationship between the dignity of death and the autonomy of human beings, and the relationship between the dignity of death and social justice. This article will also compare the Confucian views on these issues with the views of Western philosophers. Confucian ethics can offer distinct answers to the above issues and help resolve some confusions concerning concepts and theories in Western research on the dignity of death.

The development of biotechnologies has broadly interfered with a number of life processes, including human birth. An important moral question arises from the application of such medical technologies to birth: do biotechnological advancements violate human dignity? Many valid arguments have been raised. Yet bioethicists are still far from reaching a consensus on how best to protect the dignity of human birth. Confucianism is an influential ethical theory in China and presents a distinctive understanding of human dignity. In this paper, we reconstruct the two Confucian concepts of dignity—namely, universal dignity and personal dignity. We then apply these concepts to human birth in the context of biotechnologies involving embryos and genetic enhancement. We conclude that the dual Confucian understanding of dignity contributes a valuable perspective to the question of whether biotechnologies related to human birth violate human dignity.